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Eppendorf reference® – part a – chapter 8 – Eppendorf Reference User Manual

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e

Adjust control button by the volume determined.
One revolution of the control button corresponds to the following values for
water:

Clockwise rotation:

decrease in volume.

Counterclockwise rotation:

increase in volume.

f

Tighten the screw until the control button can no longer be turned.

Then continue as described in steps f and g of the Alignment Reference variable.
If the nominal volume does not correspond with the measuring result, repeat steps
d – g.
Then reseal the adjustment opening using one of the calibration seals supplied.
Alignment Reference variable:

a The pipette, tip and water must all be the same temperature

(20 – 25 °C, constant to ± 0.5 °C).

b Set the Reference variable to the desired nominal volume.

c With a pipette tip attached to the pipette, the desired volume is pipetted and

weighed 10 times. The average of this weighing is converted into μL using
the following formula:

The value obtained is the actual setting
(density of water at 20 °C: 0.9982 mg/μL; mg/μL = g/mL).

Reference fix

Vol./revol.

1, 2, 5, 10 μL

10, 20 μL

25, 50 μL

100 μL

200, 250 μL

500, 1000 μL

1500, 2000, 2500 μL

approx.

0.5 μL

approx.

1

μL

approx.

2.4 μL

approx.

5

μL

approx. 12

μL

approx. 46

μL

approx. 118

μL

Volume =

Weight

Density of liquid

(at the temperature specified)

Eppendorf Reference® – Part A – Chapter 8