Eppendorf Reference - Manual (Page 13)

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7.4 Dispensing liquid
– Hold the tip at an angle against the inside wall of the tube.
– Press down the control button slowly to the first stop (measuring stroke) and
wait until the liquid stops flowing.
– Press down the control button to the second stop (blow-out) until the tip is com-
pletely empty.
– Hold down the control button and pull the tip up the inner wall of the tube.
– Tip is ejected by pressing the control button to the final stop.
7.5
Special notes
To guarantee the highest degree of precision and accuracy, we recommend pre-
wetting all new tips by aspirating and dispensing liquid two or three times before
pipetting.
Finally, with the tip not in contact with the liquid, empty it completely on the inner
wall of the tube (via blow-out).
Explanation: Why does the pipette tip have to be pre-wetted?
To compensate for the properties of the liquid.
Wetting liquids (serum, detergent) form a thin film on the inner wall of the pipette
tip. When the first pipetting is carried out, the volume dispensed would thus be too
low.
When pipetting serum or high-viscosity solutions, wait a few seconds when
aspirating and dispensing liquid.
Eppendorf Reference® – Part A – Chapter 7