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7 operation, 1 volume setting, 2 pipette tips – Eppendorf Reference User Manual

Page 12: 3 aspirating liquid, 1 volume, Setting, 2 pipette, Tips, 3 aspirating, Liquid

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The pipette can be individually labelled. The autoclavable blank adhesive label
provided can be marked with a permanent marker and fits onto the identification
area on the top of the housing.

7.1

Volume setting

The volume is adjusted by pressing down the lateral catch and turning the control
button at the same time.
It is advisable to carry out volume setting from the higher down to the lower value.
i.e. first go above the desired volume and then return to the lower value.

7.2

Pipette tips

The pipette can function only when a pipette tip is attached into which the
liquid is aspirated.
To facilitate the search for a suitable tip, the color of the control buttons
corresponds to the color of the eppendorf tip racks.
When pipetting liquids with wetting properties different to those of water, please
observe the recommendations contained in Chapter 7.5.

7.3

Aspirating liquid

– The liquid which is to be aspirated is taken from a suitable vessel.
– Attach suitable pipette tip to the pipette firmly (observe the color coding).
– Press down the control button to the first stop (measuring stroke).
– Immerse the pipette tip vertically approx. 3 mm into the liquid.
– Allow the control button to slide back slowly.
– Pull the tip out of the liquid slowly.
– To remove any remaining droplets, dab with non-fibrous cellulose material.

When doing so, ensure that no liquid comes out of the tip.

7

Operation

Eppendorf Reference® – Part A – Chapter 7