Eppendorf Pipette Reference 2 - Manual (Page 28)

Maintenance
Eppendorf Reference
® 2
English (EN)
28
6.5 Adjusting pipettes
6.5.1 General instructions on user and factory adjustment
The Reference 2 was adjusted and tested prior to delivery. The adjustment opening is sealed using a gray
plastic adjustment seal. The adjustment seal is labeled with the abbreviation "ADJ". The adjustment display
shows "0".
Changing the adjustment of the Reference 2 is sometimes recommended for solutions that are very
different from water in terms of their density, viscosity, surface tension and/or vapor pressure, etc. If the
density of an aqueous solution changes by approximately ±10 %, for example, due to the salt
concentration, the volume changes by approximately ±0.2 %. This statement does not apply if other
relevant properties of the liquid also change.
If the pipette is used at extremely high altitudes, it must be calibrated to the ambient air pressure. At
1000 m above sea level, the volume error of a 100 μL pipette is about -0.3 %.
When using special tips, i.e. tips with a geometry that is very different from standard tips, changing the
adjustment can improve the dispensing accuracy (systematic error).
Follow the adjustment tips for epT.I.P.S. long provided in the User adjustment document on the Reference 2
CD.
Adjustment changes can be reset using simple steps.
In addition to changing the user adjustment, a Reference 2 can be permanently changed by altering the
factory adjustment.
Changes to the user or factory adjustment do not affect the dispensing precision (random error). Precision
can be improved by exchanging worn parts. Precision is also significantly affected by handling.
NOTICE! Incorrect dispensing volume due to differences in temperature and for special
liquids.
Temperature differences between the pipette, pipette tips and liquid; or solutions with
physical data that is very different from that of water; may result in incorrect dispensing
volumes.
 Avoid temperature differences the between pipette, pipette tip and liquid.
 Ensure that the temperature is between 20 °C and 27 °C and remains constant at ±0.5 °C.
 Check the dispensing volume and make sure that you can affirm all the questions listed in
the general information.
The random and systematic errors recorded on delivery can be found in the Eppendorf
Certificate. This certificate is included in delivery. Changes to the factory adjustment will
render the certificate void.