Eppendorf Pipette Reference 2 - Manual (Page 17)

Troubleshooting
Eppendorf Reference ® 2
English (EN)
17
5 Troubleshooting
5.1 Error search
Symptom/message Cause Remedy
Liquid is dripping
from the tip and/or
the dispensed
volume is incorrect. The tip is loose or the pipette tip is poorly
fitted.  Firmly attach the pipette tips. Use
epT.I.P.S. pipette tips.
Liquid with high vapor pressure and/or
different density.  Wet the tip several times and adjust
the pipette for the liquid used.
Pipetting was too quick.  Move the control button slowly.
The tip is withdrawn from the liquid too
quickly.  Withdraw the tip slowly from the
liquid with a time delay (approx. 3
seconds).
Liquid aspirated with blow-out and
dispensed with blow-out.  Repeat dispensing correctly.
Piston dirty or damaged.  Clean piston, grease slightly and/or
replace.
The tip cone is damaged.  Replace the lower part or channel.
The O-rings of the tip cones are
damaged.  Replace the O-rings (only 100 μL,
300
μL multi-channel).
The control button
jams and does not
move smoothly. • The piston is dirty.
• The seal is dirty.
• Seal defective.
• The pipette is blocked.
 Clean the lower part.
 Replace the protection filter on 2 mL
to 10 mL models.
The adjustment
seal has been
removed; the
adjustment display
has been changed. The pipette has been adjusted for a
different liquid.  Adjust the pipette for the liquid used.
No spring-loading
action of the tip
cone when pipette
tips are inserted. Spring-loading action is blocked by a
locking ring.
Spring loading is switched off
(multi-channel).  Remove the locking ring again.
 Switch on spring loading.
Use of a 2 mL to 10 mL pipette. No remedy available. The tip cone of
these sizes does not have spring-loading
action.