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2 disinfection, 3 replacing o-rings, 1 removing the o-ring – Eppendorf Pipette Reference 2 User Manual

Page 21: 2 mounting a new o-ring, Disinfection, Replacing o-rings 6.3.1, Removing the o-ring, Mounting a new o-ring

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Maintenance

Eppendorf Reference

®

2

English (EN)

21

6.2.2

Disinfection

Carefully wipe off the outer surfaces with disinfectant, DNA/RNA decontamination agents or 70 %
isopropanol.

You can wipe the outside of the tip cone and the ejector sleeve using a sodium hypochlorite solution of
4 %.

After the exposure time of the sodium hypochlorite solution (15 minutes) has elapsed, thoroughly
remove it using demineralized water.

The Reference 2 pipettes may be temporarily subjected to the UV light of a sterile bench (≥ 254 nm).

6.3

Replacing O-rings

The 100

μL

and

300

μL multi-channel lower parts are equipped with O-rings. These are wear parts. Replace

old, worn or damaged O-rings. Defective O-rings result in the incorrect positioning of the tips and in
dispensing errors.

6.3.1

Removing the O-ring

6.3.2

Mounting a new O-ring

1. Fit the mounting aid (shortened pipette tip, included in the delivery package of the O-rings) on the tip

cone.

2. Push the new O-ring over the tip onto the tip cone.

3. Check that the pipette tips are correctly positioned. Ensure that the tips are tightly sealed and properly

aligned.

1. Switch off the spring loading.

2. Push the opening of the O-ring tool (included in

the delivery package) against the tip cone from
the side so that the sharp edge in the opening of
the O-ring tool is positioned on top of the o-ring.
Do not slide the O-ring tool onto the cone!

3. Supporting the O-ring tool with your thumb, push

it firmly against the tip cone.
This O-ring is cut in one place.

4. Remove the O-ring tool and the O-ring from the

tip cone.