Eppendorf S Series 48S CO2 Incubator - Manual (Page 18)

18
CO48S-0050 Š Galaxy S Series 48 S Personal Size CO
2
Incubator User’s Guide
3.2 Preparing for Operation
1. Remove the black protective cover from the CO
2
sensor (located on the back
surface of the internal chamber), and store it for use when you clean the incubator.
There is a storage holder for the cover on the back on the incubator.
2. Ensure that the white porous sensor cover remains in place.
3. Using the power cord provided, connect the incubator to a grounded power supply.
4. Switch the incubator ON using the on/off switch at the rear of the cabinet. The
display will illuminate immediately.
5. Turn on the CO
2
gas supply with the pressure regulator set to 5 psi or 0.35 bar.
NOTE:
Be certain to check for leaks in the CO
2
connections to avoid depleting
your CO
2
gas supply. This can be accomplished using a solution of
soapy water applied to each fitting and checking for bubbles. If any
bubbles are noted, readjust the fitting.
6. The chamber setpoints are pre-programmed at 37.0°C and 5% CO
2
. Leave the
incubator on until the programmed chamber temperature and CO
2
concentration has
been reached (please see NOTE below).
NOTE:
The incubator’s CO
2
valve is disabled until the incubator reaches the
temperature setpoint. After reaching the temperature setpoint, the CO
2
valve is activated, allowing the incubator to reach the CO
2
setpoint. If
power is interrupted to the incubator long enough for the temperature to
drop below setpoint, the CO
2
valve will be deactivated until setpoint is
again achieved. (This serves to avoid spurious CO
2
readings while the
incubator is reaching its temperature setpoint)
7. Leave the incubator running for at least two hours (preferably overnight) to allow
conditions to stabilize.
3.3 Using the Humidity Tray & Humidity Control
If humidification is required, the humidity tray should be filled with 0.5 liters of warm
(
∼ 37.0ºC) distilled water at this time.
For cell culture work, we recommend the use of copper sulphate (or a recognized
biocide) in the humidity tray. Tests have shown that, in addition to inhibiting bacterial
growth in the tray, this can reduce contamination on the chamber walls. Add one small
teaspoonful (~0.11 oz or 3.2 g) of copper sulphate each time you fill the tray with fresh
water.