Eppendorf CellTram vario - Manual (Page 9)

CellTram® Air · Oil · vario — Operating manual
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2.2 Features
The CellTram Air, CellTram Oil and CellTram vario are manual piston pumps. Using a movable
piston, the CellTram generates differences in pressure which are transferred directly to a capillary
via a pressure tube. The piston is moved in the CellTram using the dial. A clockwise rotation, i.e.
to the right, results in an increase in pressure, the capillary contents are pressed out. A
counterclockwise rotation, i.e. to the left, results in a decrease in pressure, aspiration into the
capillary takes place. To assist fine pressure adjustment, the dial has a scale.
The capillary is inserted into a capillary holder which in turn provides the connection to the
pressure tube. The universal capillary holder enables permanent mounting of various capillaries
in the laboratory.
The CellTram Air, CellTram Oil and CellTram vario have a very ergonomic design which enables
easy handling, particularly in conjunction with all control units of Eppendorf micromanipulators.
2.2.1 CellTram Air
The CellTram Air uses air for pressure transmission. This guarantees clean and simple handling
of the piston pump.
A complete revolution of the dial corresponds to a change in volume of approx. 88 μL.
The position of the piston is displayed in the piston window on a scale from 0 to 15 mm
corresponding to the strokes.
2.2.2 CellTram Oil / CellTram vario
The CellTram Oil and CellTram vario use oil for pressure transmission which, unlike air, can
hardly be compressed. This ensures its operating cycles are very controlled and constant. The
simple filling technique reduces the filling time and simplifies oil filling. To ensure controlled,
constant pressure transmission, the cylinder, pressure tube and part of the capillary are filled with
oil. A complete revolution of the dial (coarse drive) corresponds to a change in volume of approx.
9.6 μL.
The CellTram vario has an additional fine drive dial. The transmission ratio between fine drive
and coarse drive is 10:1. A revolution of the fine drive corresponds to a change in volume of
approx. 0.96 μL. This enables ultra-fine cell movements.
The position of the piston can be observed in the transparent cylinder. The filling bore with the
plug screw is at the top of the cylinder. The pressure tube is connected to the CellTram Oil/
CellTram vario with the aid of the tube connection screw. The pressure tube has a joint at both
ends and thus connects the CellTram Oil/CellTram vario and the universal capillary holder.