Eppendorf CellTram vario - Manual (Page 15)

CellTram® Air · Oil · vario — Operating manual
15
4.3 Filling oil
Valid for:
•
CellTram Oil
•
CellTram vario
Abb. 9:CellTram Oil and CellTram vario
Fig. 9: CellTram Oil and CellTram vario
Hint!
Work with an air cushion in the visible area of the capillary in order to avoid contact
between the oil and the medium.
Hint!
Observe the legal regulations for purity of the oil. The supplied oil is generally used in
the ICSI area to coat drops containing embryos / egg cells or sperm. The supplied oil is
not sterile. Additional instructions can be found in the safety data sheet of the
manufacturer.
Hint!
Aspirating oil with the syringe may lead to the formation of tiny air bubbles. This should
be avoided as these air bubbles may be transferred to the CellTram, which may impair
or slow down the filling process. If the syringe contains air bubbles, these should be
collected by long-term storage. Large air bubbles can be pressed out during filling.
1 Initial position of piston 2 Filling bore with plug screw
3 Final position of piston 4 Barb screw
5 Pressure tubing connection to the rear
knurled screw
6 Grip head 0 for capillary
1 2 3 4 5 6