Eppendorf CellTram vario - Manual (Page 16)

CellTram® Air · Oil · vario — Operating manual
16
4.3.1 Heating the syringe
4.3.2 Connecting the filling tube
4.3.3 Filling the piston chamber and universal capillary holder with oil
Prerequisites
The piston is in the starting position.
1. Screw the filling hose on to the tip.
2. Aspirate at least 3 mL of oil into the syringe.
1. Turn the rotary knob to move the piston to
the initial position.
2. Unscrew the blind screw.
3. Screw the filling tube in the filling bore.
1. Hold the CellTram vertically.
2. Push some oil into the piston chamber.
During the first filling, air bubbles may be
present between the piston and the cylinder.
3. Allow these bubbles to rise by moving the
rotary knob or coarse drive back and forth.