Eppendorf Mastercycler nexus - Manual (Page 60)

PCR run
Mastercycler
® nexus
English (EN)
60
7.1.1.4 Compatible PCR plate types
The following PCR plate types are compatible. A selection of the PCR micro test tubes and lids can be found
separately (see Consumables on p. 105).
7.1.1.5 Requirements
The sample tubes must meet the following requirements:
• Temperature resistance at least 120°C
• Exact fit in the thermoblock
7.1.1.6 Maximum volumes
The possible sample volumes can vary depending on the tubes used.
For devices with an aluminum thermoblock
• PCR plates and 0.1 mL or 0.2 mL PCR tubes: max. 70 μL
• 0.5 mL PCR tubes: max. 100 μL
For devices with a silver thermoblock
• PCR plates and 0.1 mL or 0.2 mL PCR tubes: max. 100 μL
7.1.2 Inserting the sample tubes
Semi-skirted PCR plate Skirted PCR plate Non-skirted/unskirted PCR plate
PCR plate with surrounding
half-edge. PCR plate with a surrounding
edge. PCR plate without any
surrounding edge.
WARNING! Biohazard as a result of sample material directly in the thermoblock.
 Do not directly fill sample material into the thermoblock.
 Only reaction vessels, plates and lids that meet the requirements described in the
operating manual may be used.
WARNING! Biohazard due to temperature control when heated lid is open.
If the heated lid is open, the lids of the reaction vessels may come off during temperature
control. This will release sample material.
 Tempering may only be carried out when the heated lid is closed.