Eppendorf Mastercycler nexus - Manual (Page 55)

Programming
Mastercycler ® nexus
English (EN)
55
4. Press the OK softkey to accept the settings.
The program view appears.
5. Press the >> softkey. Press the Save softkey.
The changes are saved.
6. Press the Exit softkey to exit the editor.
6.3.3 Create gradient step
You can use a gradient step to optimize the specificity and yield in PCR reactions. A temperature gradient
increasing from the left to the right side of the block is generated during the holding time. The temperature
control rates before and after the gradient step are constant over the entire thermoblock.
The gradient function is only available to the following devices:
• Mastercycler nexus gradient
• Mastercycler nexus gradient eco
• Mastercycler nexus GSX1
• Mastercycler nexus GSX1e
• Mastercycler nexus flat
• Mastercycler nexus flat eco
• Mastercycler nexus GX2 (Gradient function only for thermoblock with 64 wells)
• Mastercycler nexus GX2e (Gradient function only for thermoblock with 64 wells)
Proceed as follows to define a gradient step in a PCR program:
Temp Inc./Dec. Temperature change from one cycle to the next
Adjustable from –10.0°C to +10.0°C in 0.1°C increments.
You can switch between positive and negative values by pressing the
+/-
softkey.
Gradient Temperature gradient in the thermoblock, displayed as columns
Detailed information on how to program a gradient step can be found in a
separate section (see p. 55).
Time Holding time for the program step
Time Inc./Dec. Holding time change from one cycle to the next
Adjustable from –01:00 to +01:00 in 1 s increments.
You can switch between positive and negative values by pressing the
+/-
softkey.
Ramp Temperature control speed of the thermoblock
Aluminum thermoblock: Adjustable from 0.1 °C/s to 1.5 °C/s.
Silver thermoblock: Adjustable from 0.1 °C/s to 3.0 °C/s.
Alternatively,
max. can be set. If you select max. , the maximum heating and
cooling speeds specified in the Technical Data will be used.
Details about the maximum gradient spread can be found in the product description (Tab. on
p. 19).