Agilent Technologies G1978B - Manual (Page 28)

28 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide
2 Verification
To verify sensitivity for 6130 and 6140 Quads - G1978B Multimode, Positive Scan Modes
Sensitivity Verification for Multimode in ESI, Positive Scan Mode
1 Start the ChemStation software.
2 Change the view to MSD Tune, and start an Autotune.
After the autotune completes, you may need to wait up to 30 minutes to
allow for the calibrant solution to be pumped out of the Quad instrument.
This minimizes any background signal resulting from the calibrant.
3 Change the view to Method and Run Control, and load the method
6130SCNES_MM.M (for 6130) or 6140SCNES_MM.M (for 6140).
4 Edit the method to ensure that 75:25 methanol/water with 5mM ammonium
formate is selected as the LC solvent.
5 Place the vials into the LC autosampler.
• Position #1: empty, uncapped vial
• Position #2: vial of the solvent used for dilution (solvent blank)
• Position #3: vial with the reserpine sample (10 pg/µL)
6 Run the method.
You may need to further optimize the nebulizer pressure by running FIA
experiments to achieve maximum instrument sensitivity.
The method does an FIA run with one injection of the empty vial, five
injections of the solvent blank, and five injections of the reserpine sample.
7 Review the results.
Method name 6130SCNES_MM.M
(6130 Quad)
6140SCNES_MM.M
(6140 Quad)
6130SCNCI_MM.M
(6130 Quad)
6140SCNCI_MM.M
(6140 Quad)
Performance Specification 20: 1 pk-pk
100: 1 rms
10: 1 pk-pk
50: 1 rms
Table 3 6130 and 6140 Quad Performance Verification Summary, Scan Mode
G1978B
ES Positive Scan Mode G1978B
APCI Positive Scan Mode