Agilent Technologies G1978B - Manual (Page 25)

Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 25
Verification 2
To verify sensitivity for 6130 and 6140 Quads - G1978B Multimode, Positive SIM Modes
Sensitivity Verification for Multimode in ESI, Positive SIM Mode
1 Start up the ChemStation software.
2 Change the view to MSD Tune, and start an Autotune.
After the autotune completes, you may need to wait up to 30 minutes to
allow for the calibrant solution to be pumped out of the Quad instrument.
This minimizes any background signal resulting from the calibrant.
3 Change the view to Method and Run Control, and load the method
6130SIMES_MM.M (6130 model) or 6140SIMES_MM.M (6140 model).
4 Edit the method to ensure that 75:25 methanol/water with 5mM ammonium
formate is selected as the LC solvent.
5 Place the vials into the LC autosampler.
• Position #1: empty, uncapped vial
• Position #2: vial of the solvent used for dilution (solvent blank)
• Position #3: vial with the reserpine sample (1 pg/µL)
6 Run the method.
The method does an FIA run with one injection of the empty vial, five
injections of the solvent blank, and five injections of the reserpine sample.
You may need to further optimize the nebulizer pressure by running FIA
Solvent 75:25
methanol/water
with 5mM
ammonium formate
75:25
methanol/water
with 5mM
ammonium formate
Method name 6130SIMES_MM.M
(6130 model)
6140SIMES_MM.M
(6140 model)
6130SIMCI_MM.M
(6140 model)
6140SIMCI_MM.M
(6140 model)
Performance Specification 20: 1 pk-pk
100: 1 rms
10: 1 pk-pk
50: 1 rms
Table 2 6130 and 6140 Quad Performance Verification Summary, SIM Mode
G1978B
ES Positive SIM Mode G1978B
APCI Positive SIM Mode