Agilent Technologies G1978B - Manual (Page 27)

Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 27
Verification 2
To verify sensitivity for 6130 and 6140 Quads - G1978B Multimode, Positive Scan Modes
To verify sensitivity for 6130 and 6140 Quads - G1978B Multimode,
Positive Scan Modes
Preparation
1 Transfer 1 mL of 5 ng/µL reserpine (Agilent G2423A) to a 50 mL volumetric
flask. Use a clean graduated pipette.
2 Dilute to the 50 mL mark with 75:25 methanol/water with 5 mM ammonium
formate.
3 Transfer 5 mL of the first dilution to a 50 mL volumetric flask. Use a clean
graduated pipette.
4 Dilute to the 50 mL mark with 75:25 methanol/water with 5 mM ammonium
formate.
This provides the final 10 pg/µL reserpine concentration required for
performance verification.
5 Transfer approximately 1 mL of the second dilution to an autosampler vial.
Table 3 6130 and 6140 Quad Performance Verification Summary, Scan Mode
G1978B
ES Positive Scan Mode G1978B
APCI Positive Scan Mode
Sample Reserpine, 5 ng/µL Reserpine, 5 ng/µL
Concentration after dilution 10 pg/µL 10 pg/µL
Injection volume 5 µL 5 µL
Total sample amount injected 50 pg 50 pg
Sample order number G2423A G2423A
Solvent 75:25
methanol/water
with 5mM
ammonium formate
75:25
methanol/water
with 5mM
ammonium formate