Agilent Technologies G1978B - Manual (Page 24)

24 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide
2 Verification
To verify sensitivity for 6130 and 6140 Quads - G1978B Multimode, Positive SIM Modes
When the method is finished, a report prints showing the signal-to-noise ratio
for the five blank and five sample peaks, and a blank-subtracted average of the
sample peaks. The blank-subtracted average signal-to-noise value must be
greater than or equal to 20:1 peak-to-peak (100:1 RMS).
To verify sensitivity for 6130 and 6140 Quads - G1978B Multimode,
Positive SIM Modes
Preparation
1 Transfer 1 mL of 5 ng/µL reserpine (Agilent G2423A) to a 50 mL volumetric
flask. Use a clean graduated pipette.
2 Dilute to the 50 mL mark with 75:25 methanol/water with 5 mM ammonium
formate.
3 Transfer 1mL of the first dilution to a 100 mL volumetric flask. Use a clean
graduated pipette.
4 Dilute to the 100 mL mark with 75:25 methanol/water with 5 mM
ammonium formate.
This provides the final 1 pg/µL reserpine concentration required for perfor-
mance verification.
5 Transfer approximately 1 mL of the second dilution to an autosampler vial.
Table 2 6130 and 6140 Quad Performance Verification Summary, SIM Mode
G1978B
ES Positive SIM Mode G1978B
APCI Positive SIM Mode
Sample Reserpine, 5 ng/µL Reserpine, 5 ng/µL
Concentration after dilution 1 pg/µL 1 pg/µL
Injection volume 1 µL 1 µL
Total sample amount injected 1 pg 1 pg
Sample order number G2423A G2423A