Jenway 6850 - Manual (Page 33)

33
SECTION 9 – APPENDIX
The multicell accessory measurement mode allows the user to measure a sample’s photometric
absorbance or %transmittance at up to 10 wavelengths. Following sample measurement, the
photometric readings are displayed on the multi-wavelength mode screen.
9.1 CALCULATIONS IN QUANTITATION MODE
Single Wavelength Method
：Abs.=A
1
Double Wavelengths Method
：Abs.=m*A
1
-n*A
2
Three Wavelengths Method
：Abs.=A
1
-(WL1-WL2)*(A
2
-A
3
)/(WL2-WL3)-A
3
9.2 CALCULATIONS IN DNA/PROTEIN MODE
260/280: CDNA =(A1-Aref)*f1-(A2-Aref)*f2
CProtein =(A2-Aref)*f3-(A1-Aref)*f4
Ratio
=(A1-Aref)/(A2-Aref)
A1=A260nm, A2=A280nm, Aref=A320nm (Optional)
f1=62.9, f2=36.0, f3=1550, f4=760.0
260/230:
CDNA =(A1-Aref)*f1-(A2-Aref)*f2
CProtein =(A2-Aref)*f3-(A1-Aref)*f4
Ratio
=(A1-Aref)/(A2-Aref)
A1=A260nm, A2=A230nm, Aref=A320nm (Optional)
f1=49.1, f2=3.48, f3=183, f4=75.8