Jenway 6850 User Manual

Page 29

SECTION 8 – DNA/PROTEIN

The DNA/Protein measurement mode allows the user to measure multi-wavelength absorbance ratios,

such as 260nm/280nm and 260nm/230nm, which are commonly used to estimate a protein or nucleic

acid sample’s purity. The mode also includes calculations that can be used to estimate the

concentration of the protein or nucleic acid sample.

Four commonly used protein assay methods are pre-loaded in this measurement mode. The available

protein assay methods are Direct UV, Lowry, Bradford, Biuret and BCA.

8.1 DNA/PROTEIN MODE SCREEN

Select the DNA/Protein Measurement icon

on the toolbar. The DNA/Protein measurement

window will open.

8.2 METHOD SETUP

The DNA/Protein method options are selected

from the Method drop down box. The methods

that are available are:-

1. 260/280

2. 260/230

3. UV

Method

4. Lowry

5. BCA

6. Bradford

7. Biuret

8.2.1

Adjusting the Method Parameters

Select the DNA/Protein Measurement field to

edit and enter the required wavelength or

concentration factor. The displayed

concentration unit is selected from the Unit drop

down menu.