Jenway 6850 User Manual

Page 30

8.3

DETERMINATION OF NUCLEIC ACID CONCENTRATION

Select the required method parameters as in 8.2.

8.3.1 Sample

Measurements

To quantify an unknown sample select the

Sample tab. Insert the sample cuvette into the

sample cuvette holder and the blank solution into

the reference sample holder.

Select the Start button.

A new window will open allowing the sample

name to be edited. The actual photometric

readings of the sample will be displayed in the

Readout window along with the results of the

calculations specified in the method settings.

Select OK to transfer the data to the sample

result table.

The sample result table will display the

measured absorbance values and the final

calculated concentration and ratio values.

8.4 DETERMINATION OF PROTEIN CONCENTRATION

Select the required method parameters as in 8.2.

8.4.1

Using a Concentration Factor or Pre-defined Calibration Curve

8.4.1.1 Entering Known Calibration Curve Constants

The known calibration curve constants can be

entered into the appropriate K0 – K2 fields

where:-

Concentration = (K2*X

)+ (K1*X) + K0

X = Photometric value of the sample