Jenway 6850 User Manual
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6.5.4
Spectral Points Analysis ..................................................................................................... 22
6.5.5
Spectrum Derivative ........................................................................................................... 22
6.5.6
Spectrum Smooting ............................................................................................................ 22
6.5.7
Remeasure (Re-plot) Spectrum .......................................................................................... 23
6.6
OVERLAY SPECTRA ......................................................................................................... 23
6.6.1
Spectrum Addition, Subtraction, Multiplication and Division .............................................. 23
6.6.2
Delete Displayed Spectrum ................................................................................................ 24
6.7
SPECTRUM DISPLAY AND PRINT OPTIONS .................................................................. 24
SECTION 7 – KINETICS .......................................................................................... 25
7.1
KINETICS MODE SCREEN ............................................................................................... 25
7.2
METHOD SETUP ............................................................................................................... 25
7.3
SELECTING THE MEASUREMENT MODE ...................................................................... 25
7.4
SAMPLE MEASUREMENTS .............................................................................................. 26
7.5
POST MEASUREMENT TOOLS ........................................................................................ 26
7.5.1
Adjusting the Displayed Scan Range ................................................................................. 26
7.5.2
Kinetics Zoom Function ...................................................................................................... 26
7.5.3
Spectral Points Analysis ..................................................................................................... 27
7.5.4
Kinetics Derivative .............................................................................................................. 27
7.5.5
Remeasure (Re-plot) Kinetics Scan ................................................................................... 27
7.6
OVERLAY SCANS ............................................................................................................. 27
7.6.1
Kinetics Display and Print Options ..................................................................................... 28
7.6.2
Calculate Rate of Change ................................................................................................... 28
SECTION 8 – DNA/PROTEIN .................................................................................. 29
8.1
DNA/PROTEIN MODE SCREEN ....................................................................................... 29
8.2
METHOD SETUP ............................................................................................................... 29
8.2.1
Adjusting the Method Parameters ...................................................................................... 29
8.3
DETERMINATION OF NUCLEIC ACID CONCENTRATION ............................................. 30
8.3.1
Sample Measurements ....................................................................................................... 30
8.4
DETERMINATION OF PROTEIN CONCENTRATION ...................................................... 30
8.4.1
Using a Concentration Factor or Pre-defined Calibration Curve ........................................ 30
8.4.1.1
Entering Known Calibration Curve Constants .................................................................... 30
8.4.2
Constructing a New Calibration Curve ............................................................................... 31
8.4.2.1
Measuring Standard Samples ............................................................................................ 31
8.4.3
Sample Measurement ......................................................................................................... 31
SECTION 9 – APPENDIX ........................................................................................ 33
9.1
CALCULATIONS IN QUANTITATION MODE .................................................................... 33
9.2
CALCULATIONS IN DNA/PROTEIN MODE ...................................................................... 33
SECTION 10 – TECHNICAL SUPPORT ................................................................. 34
10.1
TECHNICAL SUPPORT ..................................................................................................... 34