Jenway 6850 User Manual

Page 25

SECTION 7 – KINETICS

The kinetics measurement mode enables the absorbance or % transmittance of an active molecule to

be measured over a period of time; for example enzyme analysis of horseradish peroxidase. The

absorbance or % transmittance is measured at regular time intervals at a set wavelength over a period

of time. The results are plotted on a graph to show the change in absorbance or % transmittance over

time. Following sample measurement statistical analysis of all or part of the experiment can be

performed.

7.1 KINETICS MODE SCREEN

Select the Kinetics Measurement icon

on the toolbar. The Kinetics measurement

window will open.

7.2 METHOD SETUP

The kinetics scan settings can be edited by

selecting the settings icon in the toolbar.

The options that can be changed are:-

1. Scan

wavelength

Scan time (max 100000s)

3. Scan Step (0.1, 0.2, 0.5, 1.0, 2.0,

5.0, 10.0, 30.0, 60.0s)

The required options can be entered directly or selected from the available options via the drop down

lists. Select OK to confirm.

7.3

SELECTING THE MEASUREMENT MODE

The required measurement mode can be selected from the available options of Abs and %T by

selecting the or icons on the toolbar.