In-Situ smarTROLL Multiparameter Handheld Calibration Kit/Bubbler User Manual
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Information subject to change without notice. In-Situ, In-Situ logo, Baro Merge, BaroTROLL, HERMIT, iSitu, RDO, Pocket-Situ, RuggedCable, RuggedReader, TROLL, and Win-Situ are
trademarks or registered trademarks of In-Situ Inc. © 2012. All rights reserved.
For more information, contact In-Situ Inc.
221 East Lincoln Avenue, Fort Collins, CO 80524
1-800-446-7488 (toll-free in U.S.A. & Canada)
1-970-498-1500 (international & domestic)
www.in-situ.com
0080832 Rev. 004 04/13
Application
The RDO® Sensor is extremely stable, which eliminates
the need for frequent calibration. Standard operating
procedures, environmental conditions (e.g., potential
for fouling, abrasive conditions) may necessitate
routine calibration.
After installing a new RDO Sensor, In-Situ® recommends
that you perform a calibration. For best results, perform
a 2-point calibration at 100% and 0% dissolved oxygen
(DO) saturation before using the sensor.
100% Oxygen Saturation Calibration
1. Install the batteries in the portable air pump as
shown in the diagram on the inside cover.
2. Fill the large calibration cup to within 4 cm (1.5 in.)
of the top with clean water.
Note: Use tap water, distilled water, or your sample matrix if it
is freshwater, not brackish or saline.
3. Turn on the bubbler, open the pinch valve on the
tubing. Run at full flow rate for 10 minutes to fully
saturate the water with oxygen. After 10 minutes
you can use the control valve to match the water
turbulence conditions expected in the field.
4. Place the instrument in the water ensuring that
the sensor and the temperature thermistor are
completely submerged and that the RDO sensing
material is not in the direct path of the bubbles.
5. Connect the instrument to a computer and
establish a connection to the calibration software.
6. Follow the calibration wizard provided in the software.
Note: Video tutorials and operator’s manuals are available
on the In-Situ website www.in-situ.com in the Tech
Resources section.
0% Oxygen Saturation Calibration
1. Fill the smaller calibration cup to the upper fill line
with sodium sulfite (NA
2
SO
3
) solution.
2. Place the instrument in the solution and allow time
for temperature stabilization.
3. Ensure there are no air bubbles on the sensing
surface of the RDO Sensor.
4. Follow the calibration wizard provided in the software.
Note: Video tutorials and operator’s manuals are available
on the In-Situ website www.in-situ.com in the Tech
Resources section.
5. When you are finished rinse thoroughly in clean water.
Replacement Parts
Sodium sulfite solution, 500 mL
0017670