PASCO ME-6936 Metabolism Chamber - Manual (Page 3)

M o d e l N o . M E - 6 9 3 6 S a m p l e A c t i v i t i e s
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16. After 10 minutes, stop recording data.
17. Name the data run.
18. Remove the sensors and the dry seeds from the chamber.
19. Wave the chamber so that the air in the chamber mixes with the air in the room.
20. For the third trial, place 25 germinating seeds into the Metabolism Chamber.
21. Start data recording. Replace the sensors into the Metabolism Chamber.
22. Adjust the scale of the graphs to show all data.
23. After 10 minutes, stop recording data.
24. Name the data run.
Data Analysis
25. Apply a linear fit to the data runs to find the slope of the line.
26. Compare the rates of CO
2
production in the dry seeds versus the germinating seeds.
27. Compare the rates of O
2
consumption in the dry seeds versus the germinating seeds.
28. Compare the rates of CO
2
production and O
2
consumption for the germinating seeds.
2) AP
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Biology Lab 5: Cellular Respiration
Teacher Preparation:
• Get Alaska pea seeds (about 100 per lab group).Get glass beads approximately the same size as the
pea seeds (about 25 per lab group).
• Three to four days before the lab, soak the pea seeds in a pan of water overnight. Soak approximately
75 pea seeds per lab group.
• Twenty-four hours later, remove the pea seeds from the water and place them on a moist paper towel.
• Place the pea seeds and the towel in a plastic bag or plastic, lidded storage container and leave it in a
warm, dry place for another 24 hours. Do not seal the bag or plastic storage container.
• On the day of the lab, prepare boiled germinating pea seeds and chilled germinating pea seeds (about
25 each per lab group).
• For each lab group, put 25 germinating pea seeds into boiling water for 5 minutes. After removing
them from the water, allow them to cool, pat them dry, and place them in a beaker labeled “boiled ger-
minating pea seeds”.
• For each lab group, put 25 germinating pea seeds into a beaker labeled “chilled germinating pea
seeds”. Place the beaker in an ice water bath. Keep the beakers in a refrigerator until ready to use.
• For each lab group, put 25 germinating pea seeds into a beaker labeled “germinating pea seeds”.
• Provide each lab group with 25 dry pea seeds, 25 glass beads, 25 germinating pea seeds, 25 boiled
germinating pea seeds, and 25 chilled germinating pea seeds.