Ocean Optics Red Tide Guide - Manual (Page 10)

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Fig.27 shows a second order regression line without zero
intercept.
REACTION KINETICS –STRIP CHARTS
Strip charts plot the Y-axis value of a graph at a set wave-
length against time. For reaction kinetics the most useful
of these is Absorbance against time, which is directly pro-
portional to concentration against time.
See the SpectraSuite manual for more information.
Fig.27
1st order straight line gives a value for ε.
2nd order line gives a more accurate calibra-
tion for concentration.
IMPORTANT
Always save your calibration before clicking Finish.
Click Finish to run the concentration page. See Fig 23.
SAVING CALIBRATIONS
Running a calibration may take hours of lab work making
up solutions. It is a good idea to save your calibration
plots.
Click Save Calibration.
To run a saved calibration:
1. Go to File>New>New Concentration Measurement
2. Select Active processing if you have an absorbance
graph running or New absorbance processing if not.
3.
Click the radio button Load calibration from file (see
Fig. 21). Find the file and click Next. The regression plot
you worked so hard to create will appear.
4. Click Finish to run the concentration page (Fig.23).
CHANGING X-AXIS UNITS
The default X-axis unit is nanometres.
Go to Processing >X-axis units to change the units.
Note:
The trace usually disappears when you change units. To
recover it, click the zoom to maximum icon. See Fig.7
Note that the trendline is reversed when the X-axis unit is
an inverse of wavelength (GHz and wave number).
SOFTWARE UPDATES
SpectraSuite is updated frequently. You can download the
latest version from the Ocean Optics website
www.oceanoptics.com or use the update center in
SpectraSuite.
Daylight - Fraunhofer absorption lines can be seen as
sharp dips. Compare with reference values. Where do
these lines come from? Below a compact fluorescent
“daylight” bulb.
INTERESTING SPECTRA