Olympus IX2 RFAEVA2 - Manual (Page 20)

17
TROUBLESHOOTING GUIDE
Problem
Cause
Remedy
Caution
L
aser beam
output
No laser beam is output
.
The laser oscillator is not ON.
Set the main switch and key of the laser oscillator to ON.
Never look into an objective to check if the laser beam is output
.
The illumination selector knob is set for the mer
-
cury burner illuminator
.
Push in the illumination selector knob to select the laser illuminator light path.
The filter slider of the fiber light illuminator is pulled out
.
Push in the filter slider to open the light path.
The manual shutter of the fluor
escence mirr
or unit
cassette is closed.
Release the manual shutter
.
Other fluor
escence mirr
or unit than a total internal
reflection fluor
escence mirr
or unit is engaged in
the light path.
Engage a total internal r
eflection fluor
escence mirr
or unit
in the light path.
The laser beam incidence angle adjustment knob is turned too much counterclockwise.
Tu
rn the laser beam incidence angle adjustment knob all
the w
a
y clockwise.
The binocular tube shutter is pulled out
.
Push in the binocular tube shutter
.
Classification
It takes long time after the laser oscillator is s
w
itched ON till the laser beam is output
.
The equipment is normal if the laser beam is out- put within about 1 minute.
W
ait until the laser beam is output
.
The interlock k
e
y s
witch is set to OFF
.
Set the interlock key switch to ON.
The AC adapter connector is connected im- pr
operly
.
L
aser beam
observation
Total internal r
eflection fluor
escence cannot
be obtained.
The laser incidence angle is not the critical angle.
Turn the laser incidence angle adjustment knob counter clock
wise.
The laser beam incidence angle adjustment knob is turned too much counterclockwise.
Turn the laser beam incidence angle adjustment knob clockwise.
An objective other than the total internal r
eflection
fluorescence objectiv
e is engaged in the light path.
Engage a total internal r
eflection fluor
escence objective in
the light path.
Be sur
e to pulled out the shut
-
ter to block the laser beam be- for
e pr
oceeding to tr
eatment
.
The specimen is tilted.
Place the co
ver glass so that it is par
allel with the stage
center plate.
The specimen being observ
ed has a r
e
fr
activ
e
inde
x of 1
.38 or mor
e (gly
cerin sealed specimen,
etc.)
Use the Apo1
00X
OHR objectiv
e (NA 1
.65) to observ
e a speci-
men with a r
efr
activ
e inde
x of 1
.38 (inde
x of c
ytoplasm) or
mor
e.
The immersion oil or cover glass is not suitable.
Special immersion oil and co
ver glass ar
e requir
ed when
using the Apo1
0
0
X
OHR (NA 1
.65).
Linear or elliptical interfer
ence stripes are
observed in the field.
The ND filter in the filter slider is tilted.
Stand the ND filter vertically using the ring spring provided in the filter slider
.
If the interfer
ence stripe still does not disappear
, r
o
tate the
ND filter to minimize the interfer
ence stripe.
Be sur
e to pulled out the shut
-
ter to block the laser beam be- for
e pr
oceeding to tr
eatment
.
If the interfer
ence stripe does
not disappear after tr
eatment
,
please consult Olympus.
A fluor
escence mirr
or unit incorpor
ating an e
xcita-
tion filter is engaged in the light path.
Engage a total r
eflection fluor
escence mirr
or unit without
excitation filter in the light path.
Random interfer
ence stripe is observed in the
field, and the stripe moves when the stage is moved.
The specimen (cell) is not in close contact with the co
ver glass but is separ
ated fr
om it at some
positions.
Put the specimen (cell) in close and perfect contact with the cover glass.
The immersion oil contains bubbles.
Wipe off the immersion oil and attach new oil by avoiding bubble penetr
ation.
Random interfer
ence stripe is observ
ed in
the field, and the stripe does not move even when the stage is moved.
The
objectiv
e
is
dirt
y.
Clean the objectiv
e top lens with the cleaning mixtur
e fluid.
Connect it pr
operly
.
The specimen r
eplacement lid is r
e
m
o
ved.
Place the specimen replacement lid on the specimen box
.
An interfer
ence stripe with a concentric pat
-
tern is observed.
Dust is attached to the dichr
oic mirr
or or barrier
filter
.
Blo
w dust a
w
ay using a blo
wer
, etc.