Olympus IX2 RFAEVA2 - Manual (Page 15)

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IX2-RFAEVA-2
Fig. 2
Fig. 1
3-2 Preparation for Observation
1 Preparing for Laser Beam Introduction (Fig. 1)
2 Introducing the Laser Beam (Fig. 2)
}To ensure stable laser beam output, warm up the system (for about 10
minutes) after turning the laser power supply on.
1. Set the main switch 3 to “ I ” (ON).
2. Turn the key 4 till the ON position. This also starts the laser-cooling fans.
The laser will start oscillation in about 10 seconds to 1 minute.
3. Set the laser interlock key switch (5 in Fig. 1) to ON.
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Never peel off warning label from a position it is attached. Otherwise, the laser beam may enter your eyes.
1. Set the light path selector knob of the microscope for the camera light
path.
2. Push in the illumination selector knob @ for the laser light path.
3. Rotate the fluorescence mirror unit turret to engage an excitation mirror
unit that is compatible with total internal reflection fluorescence observation.
4. Engage the objective for total internal reflection observation in the light
path.
5. Push in the shutter of the binocular tube shutter to engage it in the light
path.
6. Push in the filter slider ² of the fiber light illuminator to engage the
released shutter.
7. Release the shutter of the fluorescence mirror unit turret (by setting the
shutter to position marked “\" ).
8. Put the specimen replacement lid on the specimen box.
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