LW Scientific Revelation lll Halogen - Manual (Page 3)

P. 770.270.1394 F. 770.270.2389 865 Marathon Parkway Lawrenceville GA 30046
Operation
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Once you have assembled all the parts and allowed your microscope to come to room temperature, plug the
power cord into the appopriate AC outlet. Note: Excess cold can fog lenses and cause lamp to fail.
Turn the light on using the black on/off switch on the right side of the base. Next adhust the light intensity using the
brightness control wheel located on the right side just behind the on/off switch. Note: Rapid, repeated changes in
light intensity will dramatically shorten the life of the quartz halogen lamp.
In order to become acquainted with the controls, choose a specimen slide with which you are familiar. For exam-
ple, an old hematology slide or a commercially prepared slide. Place the slide into the slide holder by pushing back
on the thumb guard to open the slide finger. The slide finger closes slowly to eliminate the possibility of chipping the
corner of your slide when it closes.
Move the slide to the center of the stage, by turning the stage control knobs, located just below the stage. These
knobs allow you to move the slide on the X-Y axis (forward/backward and left/right).
The sub-stage iris should then be set to match the aperture of the objective for maximum resolution under each
objective power. You should begin with the 4x or 10x objective.
4x objective 1.0 N.A. (nearly closed) 40x objective - 0.65 N.A. (halfway closed)
10x objective - 0.25 N.A. (1/4 closed) 100x objective - 1.25 N.A. (wide open)
Insert the filter of your choice into the swing-out filter holder beneath the Abbe condenser. Note that many custom-
ers prefer to use the blue filter for routine use.
Once you are comfortably seated, look into the oculars and move the eyepiece tubes together or apart until you
see only one complete circle of light. You have now adjusted your interpupillary distance.
Using the 4x or 10x objectives and the coarse and fine adjustment knobs, bring the specimen into focus. Now,
move the 40x objective into place. You will feel a “clicking” action when the objective is seated properly. Again,
adjust focus for best image. You should also adjust the iris diaphragm (as listed above) for the best contrast and
resolution.
Diopter Adjustment: Since you are using a binocular microscope, you have to adjust the normal difference in vision
between your two eyes. This is a simple but critical adjustment! Close your left eye and look into the right ocular with
your right eye. Adjust the one focus to give you the best image. Now look at the ocular tube on the left. You will see
that the left ocular tube has a built-in adjustment ring. Now close your right eye and look with your left eye into the
left ocular. Using the diopter adjustment ring on the left ocular tube, adjust the focus until you see a clear, focused
field.
Friction Adjustment: With repeated use and wear, the stage may drift out of focus. If this happens, you need only to
tighten the tension control ring (located on the right side of the microscope between the coarse adjustment and
the body of the microscope). If the coarse focus is hard to turn, you may choose to loosen the friction adjustment.
Stage Stop Lever: To help prevent the stage from hitting the objectives, the Revelation III Microscope is equipped
with an adjustable stage stop. Rotate the 100x oil objective into place, and put a slide into the slide holder. Slowly
raise the stage, stopping when the slide makes contact with the objective. Now, turn the stage stop lever in a
clockwise direction toward you. The stage stop lever is located on the left side of the microscope between the
coarse adjustment and the body of the microscope.
Revelation III Microscope will be parfocal when the diopter adjustments are both set to center (see 9 above).