Hoefer IEF100 User Manual
Page 64

• p58
Görg, A., Obermaier, C., Boguth, G., Posch, A. and Weiss, W.
Two-dimensional polyacrylamide gel electrophoresis with
immobilized pH gradients in the first dimension (IPG-Dalt):
the state of the art and the controversy of vertical vs horizontal
systems. Electrophoresis 16, 1079–1086 (1995).
Görg, A., Obermaier, C., Boguth, G., Csordas, A., Diaz, J.J., Madjar,
J.J. Very alkaline immobilized pH gradients for two dimensional
electrophoresis of ribosomal and nuclear proteins. Electrophoresis
18, 328–337 (1997).
Link, A.J. (ed) 2-D Proteome Analysis Protocols, Methods in
Molecular Biology 112 Humana Press (1998).
O’Farrell, P. H. High resolution two-dimensional electrophoresis of
proteins. J. Biol. Chem. 250, 4007–4021 (1975).
Olsson, I., Larsson, K., Palmgren, R. and Bjellqvist, B. Organic
disulfides as a means to generate streak-free two-dimensional maps
with narrow range basic immobilized pH gradient strips as first
dimension. Proteomics 2, 1630–1632 (2002).
Rabilloud, T., Use of thiourea to increase the solubility of membrane
proteins in two-dimensional electrophoresis. Electrophoresis 19,
758–760 (1998).
Rabilloud, T., Solubilization of proteins for electrophoretic analyses.
Electrophoresis 17, 813–829 (1996).
Rabilloud, T., Valette, C. and Lawrence, J.J. Sample application by
in-gel rehydration improves the resolution of two-dimensional
electrophoresis with immobilized pH gradients in the first
dimension. Electrophoresis 15, 1552–1558 (1994).
Righetti, P.G. Isoelectric Focusing: Theory, Methodology and
Applications, Elsevier, Amsterdam (1983).
Righetti, P.G. Immobilized pH gradients: theory and methodology.
Elsevier, Amsterdam (1990).
Sanchez, J.C., Rouge, V., Pisteur, M., Ravier, F., Tonella, L.,
Moosmayer, M., Wilkins, M.R. and Hochstrasser, D.F., Improved
and simplified in-gel sample application using reswelling of dry
immobilized pH gradients, Electrophoresis 18, 324–327 (1997).
Westermeier, R. and Naven, T. Proteomics in Practice, A Laboratory
Manual of Proteome Analysis, Wiley-VCH Verlag GmbH,
Weinheim (2002).