Shellab BACTROX - Manual (Page 9)

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Bactrox Operations Manual 9
5.3.2 Reading & Understanding Your CO
2
Tank Levels: Pure CO
2
is in a liquid state in the
tank, and a constant vapor pressure is generated in the tank above the liquid level.
The CO
2
is drawn off of the top as a gas. It is normal for the high pressure gauge on
your regulator to start out reading 800 to 1000 PSI with a full tank. The same vapor
pressure is maintained as long as any liquid is left in the tank. The reading will drop
from 800 to 500 PSI quickly and will stay there for most of the duration of the tank. At
the end of use, the pressure will drop quickly to zero to indicate that the tank is
completely empty. When the last of the liquid has evaporated into gas then the
pressure will drop rapidly as the gas is drawn off.
5.3.3 NOTE: Pressure from the regulator to the incubator should be set at 15 PSI. Models
with additional internal regulators have pressure reduction to 10 PSI to prevent
overshoot.
5.3.4 NOTICE: Only medical grade CO
2
should be used in your Bactrox. Failure to do so
may damage the unit and void your warranty.
NOTE: Pressure from the regulator to the incubator should be set at 15 PSI. An additional
internal regulator further reduces the pressure to 10 PSI to prevent overshoot.
5.4 Hose ports for the gas are provided on the upper left side of the unit. The Bactrox chamber has
five access ports on the left side of the unit. See Diagrams Section page 33 (INTERIOR
PLUMBING) for more details.
5.5 Install one end of the hose from the N
2
gas regulator of the gas tank to the port marked “N2
PORT ” on the plumbing connections panel.
5.6 Install one end of the hose from the CO
2
gas regulator of the gas tank to the port marked “CO2
PORT ” on then plumbing connections panel.
NOTE: CONNECTING HOSES. Hose connections are intended to be simple. Insert the hose into
the barb until the hose stops.
5.7 Plug power cord from Bactrox into a suitable outlet. Refer to the Installation Section of this
manual for additional information. Turn the power switch ON to verify power to the system.
5.8 When the power switch to the Hypoxic Chamber is activated, you should see a light in the back-
lit power switch, the O2 and CO2 digital display panels illuminate, along with a readout of the
incubator temperature. If any of these appear not to work, consult the Troubleshooting Guide.
6. THERMOELECTRIC CONDENSATE CONTROLER
6.1 The thermoelectric condensate controller collects excess humidity and eliminates chamber
condensation. Moisture is funneled into a tube for collection inside your chamber.
6.2 The thermoelectric condensate controller is located on the left side of the chamber interior
behind circulation fan. A plastic tube drains excess moisture into the working chamber, on the
back left side.
6.3 Place a container, e.g., glass flask or beaker, under the tube for collection. Empty the container
DAILY.
6.4 Desiccants are not recommended in the chamber. Desiccants are drying agents that will pull
moisture from your samples by removing humidity and leaving condensate.
7. INCUBATOR TEMPERATURE CONTROLLER/SET OVER
TEMPERATURE
Under certain circumstances, samples placed directly on the bottom shelf may become too hot and dry out. Shelf
spacers are provided to minimize this condition. Three spacers are provided for the BACTROX. Place these
spacers on the bottom shelf. Note the slots go to the front and back.
7.1 Setting Incubator Controller: The incubator temperature controller regulates the incubator
temperature by use of Up/Down buttons. To enter set point mode on the control, press either the
Up or Down button one time. The digital display will start to blink, going from bright to dim. While
blinking, the Digital Display is showing the set point. To change the set point, use the Up and
Down buttons. If the buttons are not pressed for five (5) seconds, the display will stop blinking
and will read the temperature of the unit. Note that the SET OVER TERMPERATURE knob
should be turned to its maximum position, (clockwise) until the unit has stabilized at desired set