Shellab BACTRONEZ - Manual (Page 35)

35 | P a g e
OPERATION (CONTINUED)
C HAMBER E NTRY
Perform these steps to access and work in the workspace chamber without drawing in aerobic
atmosphere. See the
Exiting the Chamber procedure on page 37 for how to withdraw your arms
from the chamber and sleeve assembly without compromising the anaerobic atmosphere.
1. Remove watches, bracelets, large rings, and any sharp objects that might damage the
cuffs or sleeves.
2. Place your hands in the sleeve assemblies so that the cuff rings are secure around the
bare skin of the widest part of your forearms.
a. The BACTRONEZ comes with mid-size size 8 cuff. Size 6 cuffs are available
for users with smaller forearms; size 9 for larger users. Please see the Parts
List on page 60.
3. Keep your hands approximately 4 – 6 inches (10 cm – 15 cm) away from the arm port
doors, and slightly to either side. This helps prevent the collapsing sleeves from pulling
your hands into the arm port doors during the next step.
4. Depress the VAC foot pedal (right). Both sleeves will deflate simultaneously. Continue
deflating until can see the definition of your fingernails through the sleeves.
5. Flush the sleeves with AMG mixture by depressing the gas foot pedal (left). Stop as soon
as you can no longer see the definition of your fingers through the sleeve, and the sleeve
material does not
cling to your forearms. Do not overfill the sleeves. “Ballooning” the
sleeves wastes gas, a nd risks compromising the sleeves’ seals at the cuff rings and arm
ports.
6. Repeat steps 4 and 5 for a minimum of three (3) complete evacuation and replacement
cycles.
7. Loosen both arm port door knobs by two or three turns.
8. Rotate the locking bars to a roughly 45° diagonal:
9. Slowly push one door in to the chamber, then the second. The Arm port doors can be hung
in the arm port door holders on the bottom of the workspace chamber incubator.
M OVING I N T HE P RESSURIZED C HAMBER
There is a minor, but noticeable feeling of resistance while working in the overpressure
environment of the workspace chamber. Moving in the chamber will temporarily increase the
chamber pressure. Large and fast movements can cause the manometer to bubble as anaerobic
atmosphere is forced out of the BACTRONEZ. Move deliberately and steadily. Withdrawing one
sleeved arm partly into the arm port while reaching in with the other (a movement combination that
looks something like swimming) can help reduce pressure and gas consumption. Additionally, use
of the vacuum foot pedal can help reduce any ballooning of the sleeves while working in the
chamber.