Gas source, Bactronez orientation, Installation (continued) – Shellab BACTRONEZ User Manual
Page 15: Bactronez, Ource, Rientation

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P a g e
INSTALLATION (CONTINUED)
G
AS
S
OURCE
Warning
: Never exceed a 5% hydrogen concentration inside the unit’s chamber.
Avertissement:
La concentration d’
hydrogène ne doit pas dépasser
5%
dans la chambre anaérobie.
The BACTRONEZ requires an anaerobic gas mixture with 5% hydrogen to drive the oxygen-
capturing catalytic process in the workspace chamber. To reduce anaerobic gas consumption
during pass box auto cycles, the BACTRONEZ may also be connected to a second compressed
gas cylinder containing an inert gas such as, nitrogen (N
2
), for use in the first two stages of the
three-stage auto cycle. Manual cycles of the pass box will only draw from the anaerobic
mixed gas cylinder attached to the GAS 1 In port.
For a single cylinder of anaerobic mixed gas (AMG), Sheldon Manufacturing recommends
5% hydrogen (H
2
), 5% Carbon Dioxide (CO
2
), and 90% Nitrogen (N
2
).
If you are using a two gas anaerobic application, Sheldon recommends one cylinder of
AMG gas at the above ratios, and a second cylinder of 100% Nitrogen (N
2
).
Gas regulators for BACTRONEZ applications will need to be set at 15 - 20 psi. Please see
on page 46 in the Operation Section for working with
units other than Pounds per Square Inch.
Anaerobic Mixed Gas is often sold by gas suppliers under the category of Anaerobic Incubation
Mixtures or Biological Atmospheres. Contact your site safety officer and review your institutional
safety protocols for handling, storing, and using compressed gasses. Follow all local ordinances
and national regulations regarding compressed gases in research, clinical, or production
environments.
BACTRONEZ
O
RIENTATION
Catalyst
Cartridge
Manometer
Workspace Chamber
Incubator
Arm Ports
Pass Box
Main Control
Panel
Gas and Vacuum
Panel