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Shellab BACTRONEZ User Manual

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TABLE OF CONTENTS

INTRODUCTION ........................................................................................................................................... 5

General Safety Considerations ................................................................................................................. 5

Engineering Improvements ....................................................................................................................... 6

Contacting Assistance ............................................................................................................................... 6

RECEIVING YOUR BACTRONEZ ............................................................................................................... 7

Inspecting the Shipment ............................................................................................................................ 7

Required and Useful Accessories ............................................................................................................. 8

Returning the Shipment ............................................................................................................................ 8

Recording Data Plate Information ............................................................................................................. 9

GRAPHIC SYMBOLS ................................................................................................................................. 10

INSTALLATION .......................................................................................................................................... 12

Ambient Conditions ................................................................................................................................. 12

Location ................................................................................................................................................... 12

UV Lighting .............................................................................................................................................. 12

High Altitude Locations ........................................................................................................................... 13

Power Source .......................................................................................................................................... 13

Lifting and Handling ................................................................................................................................ 14

Leveling ................................................................................................................................................... 14

Gas Source ............................................................................................................................................. 15

BACTRONEZ Orientation ....................................................................................................................... 15

Workspace Chamber Orientation ............................................................................................................ 16

Pass Box Orientation .............................................................................................................................. 16

Cleaning .................................................................................................................................................. 17

CONTROL PANEL OVERVIEW ................................................................................................................. 18

OPERATION ............................................................................................................................................... 22

Theory of Operation and Major Component Functions ........................................................................... 22

Setting up the BACTRONEZ ................................................................................................................... 25

Set up the Gas Supply ............................................................................................................................ 27

Single Gas Connection ........................................................................................................................... 28

Dual Gas Connection .............................................................................................................................. 28

Connecting To an In-House Vacuum System ......................................................................................... 29

Connecting the Vacuum Pump ............................................................................................................... 29

Filling the Manometer .............................................................................................................................. 29

Installing the Arm Port Doors .................................................................................................................. 29

Connecting the Foot Pedal Assembly ..................................................................................................... 30

Shelving Installation ................................................................................................................................ 31

Power the BACTRONEZ ......................................................................................................................... 31

Establishing an Anaerobic environment .................................................................................................. 32

Installing the Sleeve Assemblies ............................................................................................................. 34

Chamber Entry ........................................................................................................................................ 35

Moving In The Pressurized Chamber...................................................................................................... 35

Verifying an Anaerobic Atmosphere........................................................................................................ 36

Troubleshooting The Presence of Oxygen ............................................................................................. 37

Exiting the Chamber ................................................................................................................................ 38

Setting the Incubator Temperature ......................................................................................................... 39

Setting the Over Temperature Limit ........................................................................................................ 39

Operating the Pass Box .......................................................................................................................... 40

Loading the Workstation ......................................................................................................................... 42

Reactivating the Catalyst Cartridge......................................................................................................... 42