ChemoMetec YC-100 - Manual (Page 33)

5 Operation of the NucleoCounter
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5.3.3 External computer
If an external computer with the NucleoView software installed on it is connected, the
results and the recorded fluorescent image can be viewed using the NucleoView
software.
After the measurement is completed the cassette can be safely removed (see section
5.2). Sometimes, however, the complete process described above may be interrupted.
Please familiarize yourself with possible error handling of the NucleoCounter presented
in chapter
8 .
5.4 Result display
Following a successful analysis, the cell density will be displayed on the display screen.
Figure 13 shows an example of results as given on the display of the NucleoCounter
instrument.
Cells/ml Cells/ml Cells/ml Cells/ml: : : :
1.234 1.234 1.234 1.234 x x x x 10E6 10E6 10E6 10E6
Figure Figure Figure Figure 13 13 13 13. . . . Cell density of the cell lyzate, presented in Result mode 3.
5.4.1 Result mode
The NucleoCounter allows the results to be presented using one of three different
representations of the results. In the default display mode (mode 3) the result is
displayed with number of digits corresponding to full representation of the number of
cells per µl (with 2, 3 or 4 digits). An overview of the representations, mode 2-4, is
shown below. Mode 0 and 1 are not available.
Mode Description
2 The result is presented with two digits - ex. 1.2x10E6.
3 The result is presented with two, three or four digits - ex. 1.2x10E4,
1.23x10E5, 1.234x10E6, corresponding to a full representation of the
number of cells per µl.
4 The result is rounded off to an "even" number according to a table,
rounding-off to an "even" number generally being within a 1/2
standard deviation of repeatability (precision). NB! This presentation
does not compromise the performance of the NucleoCounter since the
rounding-off does not affect any significant digit!
To change the representation of the results follow the directions below (a letter or
number enclosed with "<" and ">" corresponds to a button on the keyboard).
•
Press <2> <1> on the keyboard of the NucleoCounter.
•
Press enter <↵>.
•
Press the mode-number of the desired representation, e.g. <3>.