5 operation of the nucleocounter yc-100 – ChemoMetec YC-100 User Manual
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5 Operation of the NucleoCounter
19
5 Operation of the NucleoCounter YC-100
5.1
Ready mode
After the NucleoCounter has started successfully the display should read:
NucleoCounter
NucleoCounter
NucleoCounter
NucleoCounter
Ready
Ready
Ready
Ready
Figure
Figure
Figure
Figure 11
11
11
11.... The NucleoCounter is ready for cell counting.
This display is also shown if the “
Esc” button is activated in the result mode or any
other situation, except during measuring or shutting down.
5.2
Inserting and removing the cassette
When a cassette has been properly loaded with lyzate mixture, the cassette is inserted
into the NucleoCounter instrument.
5.2.1
Protection of the optical system
The sample compartment lid covers the cassette insertion area. This lid is designed to
protect the area from dust and other potential contaminants, and to keep external light
from interfering with the fluorescent image recording. The lid is fitted with magnetic
hinges, so it can be easily removed for cleaning purposes.
Unless inserting/removing a cassette or cleaning the inserting area or the lid, the lid
should be kept closed at all times as means of protecting the optical system of the
NucleoCounter.
5.2.2
Inserting cassette
To insert the cassette:
1. Flip the lid up until it comes to a rest
2. Grip the cassette on either side of the piston cylinder, but taking care not to
touch the measurement windows (see Figure 12).
3. Insert the cassette, bevelled edge down, into the insertion slit
(see Figure 12).
4. Close the lid gently.