ChemoMetec YC-100 - Manual (Page 32)

5 Operation of the NucleoCounter
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After analysis the lid is lifted and the cassette is removed from the insertion slit and
disposed of according to national or regional laws or regulations regarding the nature of
the mixture it contains.
Figure Figure Figure Figure 12 12 12 12. . . . Illustration of how to grip and insert the cassette into the insertion slit
5.3 Measuring - The Run button
When a cassette has been loaded and inserted into the instrument, the measurement is
activated, by pressing the
Run button.
5.3.1 Actuator movements
When Run is pressed, the actuator moves the piston rod down through the cylinder of
the cassette. Hereby, the sample/dye mixture is transported to the cassette
measurement chamber. The form and dimensions of the flow channels facilitate
effective mixing of sample and stain. Sensors monitor sample flow. When the sample
has been correctly loaded into the measurement chamber, the piston is stopped.
5.3.2 Result presentation
The analysis will occur after a few seconds and the fluorescent image is recorded. Once
recorded, the image is analysed by the instrument’s internal software. Upon completion
of the analysis, the result is shown on the display. The entire process takes
approximately 30 seconds.