ChemoMetec YC-100 - Manual (Page 17)

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Table of contents
DECLARATION OF CONFORMITY ................................................................................ VI
WEEE DIRECTIVE INFORMATION – EUROPE ONLY ...................................................... VIII
INTRODUCTION AND INTENDED USE .......................................................................... X
WARNINGS AND PRECAUTIONS ................................................................................. 1
1 INSPECTION AND UNPACKING OF EQUIPMENT ...................................................... 7
2 INTRODUCING THE NUCLEOCOUNTER YC-100 ........................................................ 9
2.1 T HE N UCLEO C OUNTER YC-100 INSTRUMENT ............................................................ 9
2.1.1 Fluorescence microscope ........................................................................... 10
2.1.2 Built-in image analysis .............................................................................. 11
2.2 T HE N UCLEO C ASSETTE ..................................................................................... 11
2.2.1 Loading the cassette .................................................................................. 12
2.2.2 Fluorescent dye ......................................................................................... 12
2.2.3 Sample volume .......................................................................................... 12
2.2.4 Cassette handling ...................................................................................... 13
2.3 L YSIS BUFFER ............................................................................................... 13
3 CONTROL BUTTONS, KEYPAD AND DISPLAY ......................................................... 15
3.1 I NTERACTIVE C ONTROLS ................................................................................... 15
3.1.1 Control Buttons .......................................................................................... 15
3.1.2 Keypad ...................................................................................................... 15
3.2 I NTERFACE D ISPLAY ........................................................................................ 16
3.2.1 Status indicator ......................................................................................... 16
3.2.2 Display screen ........................................................................................... 16
4 INSTALLATION AND START-UP ........................................................................... 17
4.1 P OWER ON , POWER OFF ................................................................................... 17
4.1.1 Starting up ................................................................................................. 17
4.1.2 Shutting down ........................................................................................... 18
4.2 I NSTALLATION OF N UCLEO V IEW .......................................................................... 18
5 OPERATION OF THE NUCLEOCOUNTER YC-100 ...................................................... 19
5.1 R EADY MODE ................................................................................................ 19
5.2 I NSERTING AND REMOVING THE CASSETTE ............................................................... 19
5.2.1 Protection of the optical system ................................................................ 19
5.2.2 Inserting cassette ...................................................................................... 19
5.3 M EASURING - T HE R UN BUTTON ......................................................................... 20
5.3.1 Actuator movements ................................................................................. 20
5.3.2 Result presentation ................................................................................... 20
5.3.3 External computer ..................................................................................... 21
5.4 R ESULT DISPLAY ............................................................................................ 21