Bio-Rad Profinity IMAC Resins - Manual (Page 7)

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Resin Characteristics
The characteristics of Profinity IMAC resin are detailed in Table 1.
Table 1. Characteristics of Profinity IMAC Resin
* Dynamic binding capacity conditions (Q
10%
determination):
Column volume: 1 ml, (ID x H), 7 mm x 2.6 cm
Sample: 1.8 mg/ml 6xHis-tagged pure protein (32 kD)
Flow rate: 1 ml/min loading, and 2 ml/min washing and eluting
Loading buffer: 50 mM sodium phosphate, 300 mM NaCl, 5 mM imidazole, pH 8.0
Washing buffer: 50 mM sodium phosphate, 300 mM NaCl, 10 mM imidazole, pH 8.0
Elution buffer: 50 mM sodium phosphate, 300 mM NaCl, 250 mM imidazole, pH 8.0
Note: Dynamic binding capacity will vary from protein to protein.
** Maximum pressure test:
Profinity IMAC resin packed in a 1.1 x 30 cm Amicon column to a bed height of 20 cm with 20 mM sodium
phosphate buffer up to 43 psi (3 bar). Flow rates were increased stepwise by 200 cm/hr and held for 2 min
at each step. The pressure-flow curve for Profinity IMAC becomes nonlinear at pressures above 109 psi.
*** pH stability: Ligand density and protein BC are essentially unchanged after resin is treated with
pH 1–14 solutions/buffers for up to 200 hr.
Functional ligand Iminodiacetic acid (IDA)
Base bead UNOsphere base matrix
Form 50% suspension in 20% EtOH; comes precharged
with Ni
2+ or uncharged
Particle size 45–90 µm
Mean particle size 60 µm
Metal ion capacity 12–30 µmol Cu 2+ /ml Profinity IMAC resin
Dynamic binding capacity* ≥15 mg/ml resin
Recommended linear flow rate <600 cm/hr at 25°C
Maximum operating pressure** 109 psi
pH stability, uncharged resin*** (up to 200 hr) 1–14
Chemical compatibility See Table 2
Storage 4°C to ambient temperature
Shelf life in 20% EtOH >1 year at ambient temperature
Operational temperature 4–40°C
Autoclaving conditions 0.1 M sodium acetate at 120°C for 30 min
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