Bio-Rad Profinity IMAC Resins - Manual (Page 16)

12
Section 5
Column Packing —
Sample Preparation-Sized Columns
Use this method for packing Profinity ™ IMAC into small-sized sample preparation
micro spin columns.
Materials
Reagents
• 0.1 M NiSO
4
(or other suitable metal salt solution)
Note: Charging of Profinity ™ IMAC nickel-charged resin (Ni-charged resin) is not
required for initial use. For subsequent uses it is recommended that the resin be
cleaned of all contaminants, stripped of metal ions, and recharged with proper
metal ions prior to loading the next sample.
The procedure to charge Profinity IMAC (uncharged resin) is explained in Section 6,
Immobilizing Metal Ions.
Equipment
• Sample preparation-sized column (for example, Micro Bio-Spin ™
columns, catalog #732-6204)
• Plasticware, 2 ml capped and 2 ml capless tubes
• IMAC resin (Profinity IMAC or Profinity IMAC Ni-charged resin)
• Tabletop centrifuge
• 1 ml pipet with wide-bore pipet tips
Resin Preparation
Profinity IMAC and Profinity IMAC Ni-charged resins come supplied in a 20%
ethanol solution for resin storage. Before the column can be slurry packed, the
resin storage solution must be replaced with distilled water. See procedure on
page 10.
Method
1. Thoroughly suspend Profinity IMAC resin.
2. Place the column into an appropriate collection vessel and spin; for
example, 2 ml capless collection tube.
3. Using a pipet, transfer enough uncharged or Ni-charged Profinity IMAC
to a microcentrifuge tube.
If using a Micro Bio-Spin column, transfer 0.2 ml slurried uncharged or
Ni-charged Profinity IMAC to the column. This is equivalent to ~100 µl of a
packed resin bed.
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