Bio-Rad Profinity IMAC Resins - Manual (Page 13)

9
Section 4
Column Packing — Medium-Pressure
Columns
Slurry Packing an IMAC Column
Slurry packing is preferred for small columns. This method describes packing a
Profinity
™ IMAC resin with aid from a pump. For best results, 5 to 50 mm ID
columns and a bed height of 5 to 30 cm should be used.
Recommended Columns
Bio-Rad’s Bio-Scale ™ MT high-resolution columns may be used for the following
column-packing procedure. These columns are empty but may be packed with
the support of choice. Bio-Scale MT columns are convenient to use with
Bio-Rad’s BioLogic
™ system, or any medium- or high-pressure system:
• Bio-Scale MT2 column (7 x 52 mm) for bed volumes up to 2 ml
• Bio-Scale MT5 column (10 x 64 mm) for bed volumes up to 5 ml
• Bio-Scale MT10 column (12 x 88 mm) for bed volumes up to 10 ml
• Bio-Scale MT20 column (15 x 113 mm) for bed volumes up to 20 ml
Materials
Reagents
• 0.1 M NiSO
4
(or other suitable metal salt solution)
Note: Charging of Profinity IMAC nickel-charged resin (Ni-charged resin) is not
required for initial use. For subsequent uses it is recommended that the resin be
cleaned of all contaminants, stripped of metal ions, and recharged with proper
metal ions prior to loading the next sample.
The procedure to charge Profinity IMAC (uncharged resin) is explained in Section
6, Immobilizing Metal Ions.
Equipment
• Empty column (1–5 cm ID x 30 cm) with flow adaptors, inlet and outlet
ports
• Glass filter
• IMAC resin (Profinity IMAC or Profinity IMAC Ni-charged resin)
• Packing reservoir
• Pump
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