Bio-Rad Profinity IMAC Resins - Manual (Page 3)

Section 8 Preparation of Clarified E. coli Lysate
Using Denaturing Conditions ..............................18
Materials ....................................................................18
Method ......................................................................19
Section 9 Medium-Pressure Column Purification
of Histidine-Tagged (His-Tagged) Proteins
Using Nondenaturing Conditions........................21
Materials ....................................................................21
Method ......................................................................22
Section 10 Medium-Pressure Column Purification —
Using an Imidazole Gradient to
Determine Optimal Purification of
His-Tagged Proteins ..............................................23
Materials ....................................................................23
Method ......................................................................24
Section 11 Medium-Pressure Column Purification of
His-Tagged Proteins Using
Denaturing Conditions..........................................27
Materials ....................................................................27
Method ......................................................................29
Section 12 Sample Preparation-Size Spin-Column
Purification of His-Tagged Proteins
Using Nondenaturing Conditions........................31
Materials ....................................................................31
Method ......................................................................32
Section 13 Sample Preparation-Size Spin-Column
Purification of His-Tagged Proteins
Using Denaturing Conditions ..............................34
Materials ....................................................................34
Method ......................................................................35
Section 14 Batch-Mode Purification of Histidine
Proteins — Gravity Flow .......................................37
Recommended Columns............................................37
Materials ....................................................................37
Resin Preparation .......................................................38
Method ......................................................................38
Section 15 Regenerating, Cleaning, Sanitizing,
and Storage ............................................................40
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