Bio-Rad ENrich™ High-Resolution Size Exclusion Columns - Manual (Page 6)

2 ENrich SEC Instruction Manual
The ENrich support is compatible with aqueous solutions of 6 M
guanidine-HCl and 8 M urea. Detergents and organic solvents
such as methanol, ethanol, and isopropanol may also be used.
In some cases, alcohols and stability agents such as glycerol
may increase the column backpressure. Care should be taken
to maintain a flow rate at which the backpressure is below the
maximum operating pressure.
Table 1. ENrich SEC media and column characteristics.
SEC 70 SEC 650
Linear separation range, Da 500–70, 000 500–650, 000
Column dimensions,
diameter x height, mm
10 x 300 10 x 300
Column volume, ml 24 24
Nominal particle size, µm 10 ± 2 10 ± 2
Recommended flow rates, ml/min* 0.5–1.0 0.75–1.25
Maximum recommended flow rates,
ml/min*
1.5 2.0
Maximum operating pressure 600 psi,
4.1 MPa, 41 bar
600 psi,
4.1 MPa, 41 bar
Recommended sample volume, µl <250 <250
Efficiency, plates/m >20,000 >20,000
Working pH range 2–12 2–12
Operating temperatures, °C 4–40 4–40
* At room temperature. Viscosity may increase at lower temperatures, which will reduce the
recommended flow rates.
1.3 Connection to the NGC ™ and Other
Chromatography Systems
The ENrich columns are fitted with 10-32 type female fittings on either
end. Standard 10-32 fittings can be used to plumb the column to a
Bio-Rad
® NGC ™ chromatography system or another vendor’s systems.
Adaptors are available for connection to systems that use 1/4-28 fittings
(catalog # 750-0564).