Bio-Rad ENrich™ High-Resolution Size Exclusion Columns - Manual (Page 12)

8 ENrich SEC Instruction Manual
Fig. 2. Column diagram.
1. Start a slow flow rate of buffer (0.5 ml/min or less) through the
column.
2. Remove the lower end tubing and fitting from the column.
Firmly hold the bottom of the column over a sink or container.
3. Loosen the lock nut by turning it clockwise.
4. Raise the adaptor a few millimeters by slowly by turning the
adjusting nut counterclockwise.
5. Unscrew the upper retainer. Let it rest on the bottom retainer.
6. Slowly pull out the upper adaptor from the glass column. Allow
the buffer flow to maintain the integrity of the top of the bed.
7. Stop the pump. Plug the bottom of the column. Set the
column upright in a beaker. Remove the tubing and fitting from
the top of the adaptor.
8. Remove the frit from the adaptor by hooking one end of the
frit removal tool/tweezer into the frit in a sideways motion with
slight downward pressure.
9. Push the new frit into the end of the adaptor.
10. Replace the O-rings if they appear worn or torn. If the O-rings
are replaced, wet them with buffer before the next step.
11. Add a few drops of buffer to the top of the resin bed. Insert the
adaptor and push it down to the bed. Some buffer should flow
out the top of the column.
12. Screw on the upper retainer.
13. Lower the adaptor to the top of the bed by turning the
adjusting nut clockwise.
14. Tighten the lock nut by turning it counterclockwise.
Adjusting nut
Upper retainer
Lower retainer
Adaptor
Lock nut