Bio-Rad ENrich™ High-Resolution Size Exclusion Columns - Manual (Page 5)

ENrich SEC Instruction Manual 1
Section 1: Characteristics of the
ENrich
™ Size Exclusion Columns
1.1 Introduction
ENrich prepacked columns for size exclusion chromatography are
designed for rapid and reproducible high-resolution separation of
biomolecules. Each column contains a unique, high-performance
size exclusion media based on spherical polymer beads modified
to provide quick, differential size–based diffusion.
1.2 The ENrich SEC Separation Media
ENrich SEC media is available with different exclusion limits
and separation ranges. The prepacked columns contain 10 μm
beads, which produce excellent resolution of biomolecules at low
backpressures. SEC 70 is ideal for separating biomolecules up
to 70,000 Da and SEC 650 is used for larger biomolecules up to
650,000 Da.
The 10 μm particle size and narrow particle size distribution
provide excellent resolution of biomolecules at high flow rates and
with relatively low backpressures. The hydrophilic ENrich media
demonstrates extremely low nonspecific binding of biomolecules
accompanied by high recovery of biological activity.
Stability
The columns are stable over the pH range 2–12, allowing easy
cleaning and regeneration.