Bio-Rad ENrich™ High-Resolution Ion Exchange Columns - Manual (Page 5)

ENrich Instruction Manual 1
Section 1: Characteristics of the
ENrich Ion Exchange Columns
1.1 Introduction
ENrich prepacked ion exchange columns meet the needs of the
biochromatographer for rapid and reproducible high-resolution
separations of proteins and other biomolecules, including
peptides and polynucleotides. Two column sizes (bed volumes
of 1 and 8 ml) provide predictable scale-up of high-resolution
separations without sacrificing capacity.
1.2 The ENrich Separation Media
Each column contains a spherical, rigid, and highly porous
polymeric support derivatized with the strongly basic –N + (CH
3
)
3
quaternary ammonium group or the strongly acidic –SO
3
– group.
The 10 μm particle-size and narrow particle size distribution
provide excellent resolution of biomolecules at high flow rates
and with low backpressures. The hydrophilic ENrich media
demonstrate extremely low non-specific binding of biomolecules
accompanied by high recovery of biological activity.
Stability
The columns are stable over a 2–12 pH range, allowing easy
cleaning and regeneration.
The ENrich support is compatible with aqueous solutions of 6 M
guanidine-HCl and 8 M urea. Nonionic detergents and organic
solvents such as methanol, ethanol, and isopropanol may also
be used. Ionic detergents can be used only if the detergents
have the same charge as the support (that is, sodium dodecyl
sulfate (SDS), a negatively charged detergent, should not be
used with a positively charged anionic exchanger like ENrich Q).