Bio-Rad ENrich™ High-Resolution Ion Exchange Columns - Manual (Page 11)

ENrich Instruction Manual 7
Section 3 : Care of the ENrich Ion
Exchange Column
3.1 Column Cleaning
Careful preparation (especially filtration) of the sample and
the buffers will maintain the column performance and lifetime.
Normally, washing with 1.0 M NaCl or KCl will remove most
bound components. However, if there is a significant decrease in
column performance (increasing backpressures or a significant
drop in resolution), then a more extensive cleaning protocol such
as that described in steps 1–6 should be used. Always reverse
the flow during this procedure so tightly bound substances
at the top of the column are removed quickly.
During this operation do not exceed more than 50% of the
recommended flowrates (see Table 1).
1. Wash with 2 column volumes of 2.0 M NaCl or KCl followed
by 3 column volumes of water.
2. Wash with two 100 μl injections of 1.0 M NaOH followed by
3 column volumes of water.
3. Wash with two 100 μl injections of 50% acetic acid followed
by 3 column volumes of water.
4. If lipid contamination is suspected, wash with 1 column
volume of 20% ethanol followed by 3 column volumes of
water.
5. Wash with 2 column volumes of 2.0 M NaCl or KCl, or the
salt containing the desired counter-ion.
6. Wash with 3 column volumes of equilibration buffer.