Bio-Rad ENrich™ High-Resolution Ion Exchange Columns - Manual (Page 12)

8 ENrich Instruction Manual
3.2 Bed Height Adjustment
Under certain conditions of buffer composition, high flow rates
or long-term use, the resin bed may compress, creating a void
between the frit and the top of the bed. Normally, the void can
be eliminated by turning the adjusting nut clockwise until the frit
just touches the top of the bed (Figure 2). If the bed compresses
at high flow rates, stop the pump and loosen the top fitting, then
use the adjusting nut to remove the void, retighten the top fitting
and resume pumping buffer. If the bed has compressed from
long use, the top frit should be replaced as a precaution.
Fig. 2. Turning the adjusting nut raises or lowers the adaptor.