Bio-Rad ENrich™ High-Resolution Ion Exchange Columns - Manual (Page 15)

ENrich Instruction Manual 11
7. Stop the pump. Plug the bottom of the column. Set the
column upright in a beaker. Remove the tubing and fitting
from the top of the adaptor.
8. Remove the frit from the adaptor by hooking one end of the
frit removal tool/tweezer into the frit in a sideways motion
with slight downward pressure.
9. Push the new frit into the end of the adaptor.
10. Replace the O-rings if they appear worn or torn. If the
O-rings are replaced, wet them with buffer before the next
step.
11. Add a few drops of buffer to the top of the resin bed. Insert
the adaptor and push it down to the bed. Some buffer
should flow out of the top of the column.
12. Screw on the upper retainer (Figure 7).
Fig. 7. Reattach and hand-tighten the retainer.