Bio-Rad BioLogic LP System - Manual (Page 20)

e. Chart Recorder: After zeroing the UV monitor, use the thumbwheels
to set both pens near the bottom of the chart recorder paper. Set
paper speed (12 cm/hour suggested). Confirm that all switch
settings are in the position marked in green.
f. Fraction Collector: Confirm that the fraction collector is connected,
then place tubes in the fraction collector rack. Check that the drop
head is over collection tube #1. On the BioFrac, press Engage.
Section 23
Starting the Method
1. Press the RUN soft key — the system will count down 10 seconds and
then start the method.
2. When the method starts, turn the injector valve knob to the right as far
as it will go as shown in Figure 8 on the left. When the alarm sounds,
turn the valve knob to the left as shown in Figure 8 on the right.
Fig. 8.
To
Column
To
BioLogic LP
or Econo
Gradient
Pump
MV-6
Inject
Valve
Sample Loop
Waste
Inject
Port
FILL
"INJECT" POSITION
To
BioLogic LP
or Econo
Gradient
Pump
To
Column
MV-6
Inject
Valve
Sample Loop
Waste
Inject
Port
FILL
"FILL" POSITION
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