Bio-Rad BioLogic LP System - Manual (Page 19)

Fig. 7.
Section 22
Final Check Before Starting Method
1. Press the MANUAL soft key, then pres the START soft key to start the
pump.
2. Make sure that “A” buffer is selected.
3. Check the plumbing connections for leaks, check for air in the lines
and purge if necessary.
4. Press the instrument keys to check settings.
a. UV monitor: Range 0.05 AUFS. Rezero the UV monitor if
necessary. (Only zero the monitor if fluid is moving through the
flow cell.)
b. Conductivity: Minimum and maximum values match the values for
“A” and “B” buffers that were determined when purging the system.
c. Method: Press Program, then View Method — Review gradient
and fraction collector program.
d. LP Data View software: Open the LP Data View software and
press the Record button. Zero the UV monitor on the BioLogic LP
workstation, making sure that the UV trace flatlines at zero. Press
the Stop button, then the Clear button. Do not save this trace. The
software is now ready for the sample run to begin.
To
BioLogic LP
or Econo
Gradient
Pump
To
Column
MV-6
Inject
Valve
Sample Loop
Waste
Inject
Port
FILL
"FILL" POSITION
15
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