Bio-Rad Bio-Plex Pro™ Human Isotyping Assays - Manual (Page 2)

Table of Contents
Introduction 1
Principle 2
Kit Contents and Storage 4
Recommended Materials 5
Assay Workflow 6
lmportant Considerations 7
Detailed Instructions 7
1.
1. Plan Plate Layout 8
2.
2. Prepare Instrument 9
3.
3. Prepare Wash Method 10
4.
4. Prepare Wash Buffer 11
5.
5. Prepare Standards and Controls 11
6. Prepare Samples 14
7.
7. Prepare Coupled Beads 16
8.
8. Run Assay 17
9.
9. Read Plate 21
Troubleshooting Guide 28
Plate Layout Template 33
Calculation Worksheet 34
Safety Considerations 35
Legal Notices 35
Ordering Information 36